Transient transfection assays to examine the requirement of putative cell death genes.

TitleTransient transfection assays to examine the requirement of putative cell death genes.
Publication TypeJournal Article
Year of Publication1995
AuthorsOsborne BA, Smith SW, Liu ZG, McLaughlin KA, Schwartz LM
JournalMethods in cell biology
Volume46
Pagination99-106
Date Published1995
AbstractIn conclusion, this chapter provides a convenient and efficient method for the detection and analysis of transiently transfected cells. Such strategies allow a fast and simple analysis of the requirement for particular genes that have been identified as being induced during apoptosis. Our experience has been that, when screening for “cell death genes,” it is easy to isolate genes induced during apoptosis but far more difficult to determine the requirement for any given gene. These protocols have rendered such determinations much simpler to perform.
Alternate JournalMethods Cell Biol.