Identification and functional analysis of an ovarian form of the egg activation factor phospholipase C zeta (PLCζ) in pufferfish.

TitleIdentification and functional analysis of an ovarian form of the egg activation factor phospholipase C zeta (PLCζ) in pufferfish.
Publication TypeJournal Article
Year of Publication2011
AuthorsCoward K, Ponting CP, Zhang N, Young C, Huang C-J, Chou C-M, Kashir J, Fissore RA, Parrington J
JournalMolecular reproduction and development
Volume78
Issue1
Pagination48-56
Date Published2011 Jan
ISSN1098-2795
KeywordsAnimals, Base Sequence, Calcium, Chickens, Female, Fish Proteins, Gene Expression Regulation, Enzymologic, Humans, Male, Mice, Molecular Sequence Data, Organ Specificity, Ovary, Ovum, Phosphoinositide Phospholipase C, Species Specificity, Takifugu
AbstractRecent studies suggest that egg activation in mammals is triggered by a sperm-specific phospholipase C, PLCzeta. In other vertebrate species such as medaka fish, chickens, and quail, PLCzeta is also expressed as a testis-specific mRNA. Functional studies suggest that PLCzeta plays a similar role as a trigger of egg activation in these species. Here, we report the identification of PLCzeta orthologues in pufferfish species Takifugu rubripes (Fugu) and Tetraodon nigroviridis (Tetraodon). Unexpectedly in these species PLCzeta is expressed not in the testis, but in ovary and brain. Injection of pufferfish PLCzeta copy ribonucleic acid (cRNA) into mouse eggs failed to trigger calcium oscillations, unlike medaka PLCzeta cRNA. Our findings provide the first evidence that PLCzeta may be expressed in the egg, rather than the sperm, in some vertebrate species, and that its mechanism of action and physiologic role at fertilization may differ in different vertebrate species.
DOI10.1002/mrd.21262
Alternate JournalMol. Reprod. Dev.
PubMed ID21268182