Detection and differentiation of normal, cancerous, and metastatic cells using nanoparticle-polymer sensor arrays.

TitleDetection and differentiation of normal, cancerous, and metastatic cells using nanoparticle-polymer sensor arrays.
Publication TypeJournal Article
Year of Publication2009
AuthorsBajaj A, Miranda OR, Kim I-B, Phillips RL, Jerry JD, Bunz UHF, Rotello VM
JournalProceedings of the National Academy of Sciences of the United States of America
Volume106
Issue27
Pagination10912-6
Date Published2009 Jul 7
AbstractRapid and effective differentiation between normal and cancer cells is an important challenge for the diagnosis and treatment of tumors. Here, we describe an array-based system for identification of normal and cancer cells based on a “chemical nose/tongue” approach that exploits subtle changes in the physicochemical nature of different cell surfaces. Their differential interactions with functionalized nanoparticles are transduced through displacement of a multivalent polymer fluorophore that is quenched when bound to the particle and fluorescent after release. Using this sensing strategy we can rapidly (minutes/seconds) and effectively distinguish (i) different cell types; (ii) normal, cancerous and metastatic human breast cells; and (iii) isogenic normal, cancerous and metastatic murine epithelial cell lines.
Alternate JournalProc. Natl. Acad. Sci. U.S.A.