Complete globozoospermia associated with PLCζ deficiency treated with calcium ionophore and ICSI results in pregnancy.

TitleComplete globozoospermia associated with PLCζ deficiency treated with calcium ionophore and ICSI results in pregnancy.
Publication TypeJournal Article
Year of Publication2010
AuthorsTaylor SL, Yoon SY, Morshedi MS, Lacey DR, Jellerette T, Fissore RA, Oehninger S
JournalReproductive biomedicine online
Volume20
Issue4
Pagination559-64
Date Published2010 Apr
ISSN1472-6491
KeywordsAcrosome, Adult, Calcium, DNA Fragmentation, Embryo Transfer, Female, Humans, Infertility, Male, Ionophores, Male, Phosphoinositide Phospholipase C, Pregnancy, Semen Analysis, Sperm Injections, Intracytoplasmic, Spermatozoa, Treatment Outcome
AbstractGlobozoospermia is an infrequent pathology in which spermatozoa lack acrosomes. Patients are considered sterile without IVF augmented with intracytoplasmic sperm injection (ICSI), as fertilization is impaired due to absence of oocyte activation. As far as is known, this is the first study to report results of a comprehensive approach to the treatment of the semen parameters, sperm DNA fragmentation, aneuploidy, transmission electron microscopy, Western blotting and immunofluorescence for detection of phospholipase C zeta (PLCzeta), as well as ICSI outcome, of an affected patient. Morphological evaluation and transmission electron microscopy revealed complete globozoospermia with significant duplicate heads and tails. Analysis for DNA damage revealed fragmentation rates of approximately 80% in semen and 15-23% in swim-up fractions. PLCzeta was not detected by immunofluorescence or Western blotting. Aneuploidy rates were within normal ranges. ICSI followed by oocyte activation with calcium ionophore resulted in high rates of fertilization, and an ongoing pregnancy was established after transfer of cryopreserved-thawed embryos.
DOI10.1016/j.rbmo.2009.12.024
Alternate JournalReprod. Biomed. Online
PubMed ID20133201