Cell death suffers a TKO.

TitleCell death suffers a TKO.
Publication TypeJournal Article
Year of Publication1995
AuthorsOsborne BA, Schwartz LM
JournalBioEssays : news and reviews in molecular, cellular and developmental biology
Volume17
Issue6
Pagination557-9
Date Published1995 Jun
AbstractThe cytokine interferon-gamma (IFN-gamma), initiates both cell cycle arrest and cell death in certain cell lines. Through a novel strategy of cell transfection with episomal vectors expressing antisense cDNAs, Deiss et al. have demonstrated that it is possible to isolate genes that are required for the initiation of cell death by the cytokine IFN-gamma. This approach, referred to as TKO, for Technical Knock Out, has identified several genes whose activity appears to be essential for the induction of apoptosis by IFN-gamma in HeLa cells. Interestingly, these genes appear to mediate IFN-gamma-induced apoptosis in HeLa cells, but their inhibition by antisense does not ameliorate the antiproliferative effects of IFN-gamma in these cells. The clever strategy employed by these authors holds promise for others who wish to isolate genes required for other differentiative processes in cultured cell lines.
Alternate JournalBioessays