| Title | Activation of bovine peripheral blood gammadelta T cells for cell division and IFN-gamma production. |
| Publication Type | Journal Article |
| Year of Publication | 2002 |
| Authors | Baldwin CL, Sathiyaseelan T, Naiman B, White AM, Brown R, Blumerman S, Rogers A, Black SJ |
| Journal | Veterinary immunology and immunopathology |
| Volume | 87 |
| Issue | 3-4 |
| Pagination | 251-9 |
| Date Published | 2002 Sep 10 |
| Abstract | Bovine peripheral blood gammadelta T cells have been evaluated for effector function (IFN-gamma production) and clonal expansion in a variety of systems including following activation by mitogens, IL-12, and stimulation, through the T cell receptor (TCR) with anti-CD3 monoclonal antibody (mAb), a cell-bound molecule and a soluble antigenic extract. To evaluate cell division, carboxyfluorescein succinimidyl ester (CFSE) loading of cells and flow cytometric analysis were used, while IFN-gamma production was evaluated by intracytoplasmic staining. It was found that bovine gammadelta T cells produced IFN-gamma and clonally expanded when stimulated through the TCR/CD3 complex by a cell-associated autologous molecule on monocyte, by bacterial components following in vivo sensitization of gammadelta T cells with a leptospira vaccine or by anti-CD3 mAb. In addition, gammadelta T cells were activated efficiently for effector function but not clonal expansion by culturing with IL-12. In contrast, stimulation by Con A or PMA/ionomycin induced efficient replication but only low level IFN-gamma production which was not enhanced by the presence of IL-12. In several systems the amount of IFN-gamma produced per cell by gammadelta T cells was less than that produced by CD4 T cells in the same cultures. |
| Alternate Journal | Vet. Immunol. Immunopathol. |
