αβ T cell receptors that do not undergo major histocompatibility complex-specific thymic selection possess antibody-like recognition specificities.

Titleαβ T cell receptors that do not undergo major histocompatibility complex-specific thymic selection possess antibody-like recognition specificities.
Publication TypeJournal Article
Year of Publication2012
AuthorsTikhonova AN, Van Laethem F, Hanada K-ichi, Lu J, Pobezinsky LA, Hong C, Guinter TI, Jeurling SK, Bernhardt G, Park J-H, Yang JC, Sun PD, Singer A
JournalImmunity
Volume36
Issue1
Pagination79-91
Date Published2012 Jan 27
ISSN1097-4180
KeywordsAmino Acid Sequence, Animals, Antibody Specificity, Epitopes, T-Lymphocyte, Gene Deletion, Ligands, Major Histocompatibility Complex, Mice, Molecular Sequence Data, Protein Binding, Receptors, Antigen, T-Cell, alpha-beta, Receptors, Virus, T-Lymphocytes, Thymus Gland
Abstract

Major histocompatibility complex (MHC) restriction is the cardinal feature of T cell antigen recognition and is thought to be intrinsic to αβ T cell receptor (TCR) structure because of germline-encoded residues that impose MHC specificity. Here, we analyzed αβTCRs from T cells that had not undergone MHC-specific thymic selection. Instead of recognizing peptide-MHC complexes, the two αβTCRs studied here resembled antibodies in recognizing glycosylation-dependent conformational epitopes on a native self-protein, CD155, and they did so with high affinity independently of MHC molecules. Ligand recognition was via the αβTCR combining site and involved the identical germline-encoded residues that have been thought to uniquely impose MHC specificity, demonstrating that these residues do not only promote MHC binding. This study demonstrates that, without MHC-specific thymic selection, αβTCRs can resemble antibodies in recognizing conformational epitopes on MHC-independent ligands.

DOI10.1016/j.immuni.2011.11.013
Alternate JournalImmunity
PubMed ID22209676