Defining the Role of RBBP4 in Oocyte Maturation and Preimplantation Development using Trim-away

Holly Barletta, MS Candidate in the Cui Lab will present her Master’s Thesis on Tuesday, May 11th, 4-5 PM. Join via Zoom.

Based on a previous collaboration between the Mager Lab and the Cui lab (https://doi.org/10.1093/biolre/ioaa046), RBBP4 was shown to be essential for mouse early embryogenesis. To expand the knowledge of RBBP4 function during oocyte maturation and preimplantation development, Trim-Away, which is a new, acute, and endogenous protein depletion technique that can overcome limitations of CRISPR/Cas9 and RNAi, was applied in this project. Using Trim-Away offers opportunities to study new functions of RBBP4 and its maternal effect, and increase the knowledge on this new protein depletion technique.