"Shedding light on equine laminitis: Proteomic and histologic studies of the hoof lamellar microanatomy"

Shedding light on equine laminitis: Proteomic and histologic studies of the hoof lamellar microanatomy”

Hanna Galantino-Homer, VMD,PhD
UPenn
Wed, 2/18/2015 - 4:00pm

221 Integrated Sciences Building

Seminar Abstract
The epidermal-dermal interface between the equine hoof capsule and underlying dermal corium is a key element of the suspensory apparatus of the distal phalanx, is formed by hundreds of interdigitating primary and secondary dermal and epidermal lamellae, and undergoes damage and failure in horses afflicted with laminitis. My laboratory is interested in the microanatomical, cellular, and molecular events of laminitis pathogenesis. An initial discovery mode quantitative proteomic study of lamellar tissue from an experimental induction model of laminitis resulted in the identification of a tissue-specific, novel keratin isoform pair and several differentially expressed proteins. The identified differentially expressed proteins are consistent with histologic evidence of altered tissue differentiation and cell stress during laminitis pathogenesis. In particular, we have examined changes in the abundance and localization of the cytoskeletal intermediate filament protein, keratin-14, and the cytolinker protein, desmoplakin. Our recent development of a lectin-based fluorescent counterstain for equine lamellar tissue has allowed us to more precisely localize changes in the expression and localization of keratin-14, desmoplakin, and other structural and adhesion proteins in laminitic and control horses. Altered keratin-14 expression correlates with changes in desmoplakin subcellular localization. These studies contribute to building evidence that the epidermal lamellae play an integral role in laminitis pathogenesis and the mechanical failure of the digit.

Dr. Galantino-Homer is a graduate of Swarthmore College and the Veterinary Medical Scientist Training Program at the University of Pennsylvania. She received her veterinary degree from the School of Veterinary Medicine in 1993 and her doctor of philosophy degree in Cell and Developmental Biology from Biomedical Graduate Studies in 2000 under the mentorship of Greg Kopf of the Center for Research on Reproduction and Women’s Health on signaling events associated with bovine sperm capacitation. She continued studies of bovine and porcine sperm capacitation, cryopreservation, and cryocapacitation in Ina Dobrinski’s laboratory at the Center for Animal Transgenesis and Germ Cell Research at New Bolton Center and also became a diplomate of the American College of Theriogenologists in 2004. Dr. Galantino-Homer was appointed Senior Research Investigator in Laminitis at New Bolton Center in 2007 and has established a basic research laboratory for molecular and in vitro investigations of laminitis pathogenesis.

Notes: 

Refreshments at 3:45pm

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