Richard A. Goldsby

Richard A. Goldsby

Adjunct Professor of Veterinary & Animal Sciences

Professor of Biology, Amherst College

Photo of Richard Goldsby

Office phone: 413-542-8084

Email: ragoldsby [at] amherst [dot] edu

Ph.D.: University of California at Berkeley, 1961
Honors: John Woodruff Simpson Lectureship

Classes:
ANIML SCI 572 - Infection and Immunity
ANIML SCI 697K - Advanced Immunology
ANIML SCI 797M - Molecular and Cellular Biology of Cancer


  

Somatic Hypermutation of Immunoglobulin Genes; Immunoglobulin Diversification in Cattle

Diversity is the hallmark of the immune system and somatic mutation generates significant antibody diversity in a number of species including humans, rodents, and cattle. The mechanism responsible for the somatic hypermutation of antibody genes remains one of the unsolved problems of immunology. Work in my laboratory is directed toward the establishment of cell culture systems that will allow the observation and analysis of this process in vitro. Because the ileal Peyer’s patch (IPP) of cattle is the site of massive (involving up to >1X10E11 B cells) developmentally regulated, extensive somatic diversification of antibody genes, it is a principal target of our studies. In collaboration with the Osborne laboratory, we conduct a continuing program to understand the basic mechanisms of antibody diversification in ruminants and to characterize the bovine immunoglobulin heavy and light immunoglobulin loci.

Tilahun ME, Kwan A, Natarajan K, Quinn M, Tilahun AY, Xie C, Margulies DH, Osborne BA, Goldsby RA, Rajagopalan G.  2011.  Chimeric anti-staphylococcal enterotoxin B antibodies and lovastatin act synergistically to provide in vivo protection against lethal doses of SEB.. PloS one. 6(11):e27203.
Tilahun ME, Rajagopalan G, Shah-Mahoney N, Lawlor RG, Tilahun AY, Xie C, Natarajan K, Margulies DH, Ratner DI, Osborne BA et al..  2010.  Potent neutralization of staphylococcal enterotoxin B by synergistic action of chimeric antibodies.. Infection and immunity. 78(6):2801-11.
Tonomura N, McLaughlin K, Grimm L, Goldsby RA, Osborne BA.  2003.  Glucocorticoid-induced apoptosis of thymocytes: requirement of proteasome-dependent mitochondrial activity.. Journal of immunology (Baltimore, Md. : 1950). 170(5):2469-78.
Jeong Y, Osborne BA, Goldsby RA.  2001.  Early Vlambda diversification in sheep.. Immunology. 103(1):26-34.
Lucier MR, Thompson RE, Waire J, Lin AW, Osborne BA, Goldsby RA.  1998.  Multiple sites of V lambda diversification in cattle.. Journal of immunology (Baltimore, Md. : 1950). 161(10):5438-44.
Meyer A, Parng CL, Hansal SA, Osborne BA, Goldsby RA.  1997.  Immunoglobulin gene diversification in cattle.. International reviews of immunology. 15(3-4):165-83.
Lin AW, Usera MA, Barrett TJ, Goldsby RA.  1996.  Application of random amplified polymorphic DNA analysis to differentiate strains of Salmonella enteritidis.. Journal of clinical microbiology. 34(4):870-6.
Parng CL, Hansal S, Goldsby RA, Osborne BA.  1996.  Gene conversion contributes to Ig light chain diversity in cattle.. Journal of immunology (Baltimore, Md. : 1950). 157(12):5478-86.
Osborne BA, Smith SW, McLaughlin KA, Grimm L, Morgan G, Goldsby RA.  1996.  Genetic regulation of apoptosis in the mouse thymus.. Advances in experimental medicine and biology. 406:199-207.
McLaughlin KA, Osborne BA, Goldsby RA.  1996.  The role of oxygen in thymocyte apoptosis.. European journal of immunology. 26(5):1170-4.
Parng CL, Hansal S, Goldsby RA, Osborne BA.  1995.  Diversification of bovine lambda-light chain genes.. Annals of the New York Academy of Sciences. 764:155-7.
Ravichandran KS, Semproni AR, Goldsby RA, Osborne BA.  1992.  Immunoglobulin VH usage analysis by fluorescent in situ hybridization and flow cytometry.. Journal of immunological methods. 153(1-2):249-59.
Lin AW, Goldsby RA, Snoeyenbos GH.  1992.  Salmonella enteritidis-specific monoclonal antibodies.. Avian diseases. 36(2):455-8.
Goldsby RA, Srikumaran S, Arulanandam A, Hague B, Ponce de Leon FA, Sevoian M, Guidry AJ.  1987.  The application of hybridoma technology to the study of bovine immunoglobulins.. Veterinary immunology and immunopathology. 17(1-4):25-35.