Virus-inspired approach to nonviral gene delivery vehicles.

TitleVirus-inspired approach to nonviral gene delivery vehicles.
Publication TypeJournal Article
Year of Publication2009
AuthorsRoy R, Jerry JD, Thayumanavan S
JournalBiomacromolecules
Volume10
Issue8
Pagination2189-93
Date Published2009 Aug 10
ISSN1526-4602
KeywordsBreast Neoplasms, Cells, Cultured, Female, Gene Products, tat, Gene Transfer Techniques, Genetic Vectors, Humans, Kidney, Peptide Fragments, Polymers, Transfection
Abstract

The basic TAT peptide, responsible for translocation of the HIV-TAT protein, has been conjugated to a variety of artificial nanoscopic materials to transport them across the cellular membrane. However, attempts to translocate genes using the TAT-peptide had met with limited success. We hypothesized that the cationic nature of the peptide does not allow for displaying these peptides on the surface of the polyplex. To circumvent this potential issue, we have developed a new molecular design strategy where the TAT-peptide can be effectively displayed on the surface of the polyplex, thus enhancing gene expression.

DOI10.1021/bm900370p
Alternate JournalBiomacromolecules
PubMed ID19722558