Transgenic bovine chimeric offspring produced from somatic cell-derived stem-like cells.

TitleTransgenic bovine chimeric offspring produced from somatic cell-derived stem-like cells.
Publication TypeJournal Article
Year of Publication1998
AuthorsCibelli JB, Stice SL, Golueke PJ, Kane JJ, Jerry J, Blackwell C, Ponce de León FA, Robl JM
JournalNat Biotechnol
Volume16
Issue7
Pagination642-6
Date Published1998 Jul
ISSN1087-0156
KeywordsAnimals, Animals, Genetically Modified, beta-Galactosidase, Blastocyst, Cattle, Cell Differentiation, Cell Fusion, Chimera, Ectoderm, Embryo Transfer, Endoderm, Gene Transfer Techniques, Genetic Therapy, Mesoderm, Stem Cells
Abstract

We have developed a method, using nuclear transplantation, to produce transgenic embryonic stem (ES)-like cells from fetal bovine fibroblasts. These cells, when reintroduced into preimplantation embryos, differentiated into derivatives from the three embryonic germ layers, ectoderm, mesoderm, and endoderm, in 5-month-old animals. Six out of seven (86%) calves born were found to be chimeric for at least one tissue. These experiments demonstrate that somatic cells can be genetically modified and then de-differentiated by nuclear transfer into ES-like cells, opening the possibility of using them in differentiation studies and human cell therapy.

DOI10.1038/nbt0798-642
Alternate JournalNat. Biotechnol.
PubMed ID9661197