Strategies for dissecting epigenetic mechanisms in the mouse.

TitleStrategies for dissecting epigenetic mechanisms in the mouse.
Publication TypeJournal Article
Year of Publication2005
AuthorsMager J, Bartolomei MS
JournalNature genetics
Volume37
Issue11
Pagination1194-200
Date Published2005 Nov
ISSN1061-4036
KeywordsAnimals, DNA Methylation, Epigenesis, Genetic, Gene Expression Regulation, Genes, Tumor Suppressor, Genome, Genomics, Mice, Models, Genetic, Mutagenesis, Phenotype, Proteomics
AbstractEpigenetics generally refers to heritable changes in gene expression that are independent of nucleotide sequence. With complete genome sequences in hand, understanding the epigenetic control of genomes is the next step towards comprehending how the same DNA sequence gives rise to different cells, lineages and organs. Epigenetics also contributes to individual variation in normal biology and in disease states. The mouse provides a unique opportunity to understand how epigenetic differences contribute to both development and disease in a tractable mammalian system. Here we discuss current approaches and protocols used to study epigenetics in the mouse, including loss-of-function studies, mutagenesis screens, somatic cell nuclear transfer, genomics and proteomics.
DOI10.1038/ng1664
Alternate JournalNat. Genet.
PubMed ID16254566