Sperm phosphoproteomics: historical perspectives and current methodologies.
|Title||Sperm phosphoproteomics: historical perspectives and current methodologies.|
|Publication Type||Journal Article|
|Year of Publication||2012|
|Authors||Porambo JR, Salicioni AM, Visconti PE, Platt MD|
|Journal||Expert Rev Proteomics|
|Date Published||2012 Oct|
|Keywords||Fertilization, Gene Expression Regulation, Humans, Infertility, Male, Male, Mass Spectrometry, Phosphoproteins, Phosphorylation, Proteomics, Spermatozoa|
Mammalian sperm are differentiated germ cells that transfer genetic material from the male to the female. Owing to this essential role in the reproductive process, an understanding of the complex mechanisms that underlie sperm function has implications ranging from the development of novel contraceptives to the treatment of male infertility. While the importance of phosphorylation in sperm differentiation, maturation and fertilization has been well established, the ability to directly determine the sites of phosphorylation within sperm proteins and to quantitate the extent of phosphorylation at these sites is a recent development that has relied almost exclusively on advances in the field of proteomics. This review will summarize the work that has been carried out to date on sperm phosphoproteomics and discuss how the resulting qualitative and quantitative information has been used to provide insight into the manner in which protein phosphorylation events modulate sperm function. The authors also present the proteomics process as it is most often utilized for the elucidation of protein expression, with a particular emphasis on the way in which the process has been modified for the analysis of protein phosphorylation in sperm.
|Alternate Journal||Expert Rev Proteomics|