Somatic cell cloned transgenic bovine neurons for transplantation in parkinsonian rats.

TitleSomatic cell cloned transgenic bovine neurons for transplantation in parkinsonian rats.
Publication TypeJournal Article
Year of Publication1998
AuthorsZawada WM, Cibelli JB, Choi PK, Clarkson ED, Golueke PJ, Witta SE, Bell KP, Kane J, Ponce de Leon FA, Jerry DJ, Robl JM, Freed CR, Stice SL
JournalNat Med
Volume4
Issue5
Pagination569-74
Date Published1998 May
ISSN1078-8956
KeywordsAnimals, Animals, Genetically Modified, Cattle, Cloning, Organism, Dopamine, Embryonic Structures, Lac Operon, Mesencephalon, Neurons, Parkinson Disease, Rats, Transplantation, Heterologous
Abstract

Parkinson’s disease symptoms can be improved by transplanting fetal dopamine cells into the putamen of parkinsonian patients. Because the supply of human donor tissue is limited and variable, an alternative and genetically modifiable non-human source of tissue would be valuable. We have generated cloned transgenic bovine embryos, 42% of which developed beyond 40 days. Dopamine cells collected from the ventral mesencephalon of the cloned fetuses 42 to 50 days post-conception survived transplantation into immunosuppressed parkinsonian rats and cells from cloned and wild-type embryos improved motor performance. Somatic cell cloning can efficiently produce transgenic animal tissue for treating parkinsonism.

Alternate JournalNat. Med.
PubMed ID9585230