Molecular cloning of bovine chemokine receptors and expression by WC1+ gammadelta T cells.

TitleMolecular cloning of bovine chemokine receptors and expression by WC1+ gammadelta T cells.
Publication TypeJournal Article
Year of Publication2007
AuthorsBlumerman SL, Wang F, Herzig CTA, Baldwin CL
JournalDevelopmental and comparative immunology
Volume31
Issue1
Pagination87-102
Date Published2007
AbstractChemokine receptors mediate leukocyte migration into secondary lymphoid tissues and localization to peripheral inflammation sites. We describe full-length cDNA sequences of bovine chemokine receptors CCR5, CCR7, CXCR3 and CXCR5 and transcript expression by WC1(+)gammadelta T cells, a unique cell population with proinflammatory characteristics that comprises a large proportion of mononuclear cells in young ruminants. Bovine chemokine sequences were more similar to those of humans than were murine sequences to humans’, ranging from 84% to 91%. Transcript analysis showed that antigen stimulation of WC1(+)gammadelta T cells induced IFN-gamma production and substantially increased CCR5 and CXCR3 expression when compared with freshly isolated (ex vivo) cells. CCR7 transcripts were minimally expressed in ex vivo and proliferating WC1(+)gammadelta T cells and CXCR5 expression was negligible. These results confirm the proinflammatory nature of WC1(+)gammadelta T cells is reflected by its chemokine receptor expression and suggest WC1(+)gammadelta T cells are unlikely to transit through secondary lymphoid tissues.
Alternate JournalDev. Comp. Immunol.