Mechanism of a genetically encoded dark-to-bright reporter for caspase activity.

TitleMechanism of a genetically encoded dark-to-bright reporter for caspase activity.
Publication TypeJournal Article
Year of Publication2011
AuthorsNicholls SB, Chu J, Abbruzzese G, Tremblay KD, Hardy JA
JournalThe Journal of biological chemistry
Volume286
Issue28
Pagination24977-86
Date Published2011 Jul 15
ISSN1083-351X
KeywordsAnimals, Apoptosis, Caspases, Catalysis, Genes, Reporter, Green Fluorescent Proteins, Mice, Microscopy, Fluorescence, NIH 3T3 Cells, Proteolysis
AbstractFluorescent proteins have revolutionized modern biology with their ability to report the presence of tagged proteins in living systems. Although several fluorescent proteins have been described in which the excitation and emission properties can be modulated by external triggers, no fluorescent proteins have been described that can be activated from a silent dark state to a bright fluorescent state directly by the activity of an enzyme. We have developed a version of GFP in which fluorescence is completely quenched by appendage of a hydrophobic quenching peptide that tetramerizes GFP and prevents maturation of the chromophore. The fluorescence can be fully restored by catalytic removal of the quenching peptide, making it a robust reporter of proteolysis. We have demonstrated the utility of this uniquely dark state of GFP as a genetically encoded apoptosis reporter that monitors the function of caspases, which catalyze the fate-determining step in programmed cell death. Caspase Activatable-GFP (CA-GFP) can be activated both in vitro and in vivo, resulting in up to a 45-fold increase in fluorescent signal in bacteria and a 3-fold increase in mammalian cells. We used CA-GFP successfully to monitor real-time apoptosis in mammalian cells. This dark state of GFP may ultimately serve as a useful platform for probes of other enzymatic processes.
DOI10.1074/jbc.M111.221648
Alternate JournalJ. Biol. Chem.
PubMed ID21558267