Dissecting DR3 signaling.

TitleDissecting DR3 signaling.
Publication TypeJournal Article
Year of Publication2014
AuthorsPobezinskaya YL, Liu Z, Choksi S
JournalMethods Mol Biol
Volume1155
Pagination15-22
Date Published2014
ISSN1940-6029
Abstract

Receptor signaling can be evaluated in multiple ways, including analysis of phosphorylation of downstream molecules and analysis of proteins that are recruited to the receptor upon ligand binding. Majority of studies on the mechanism of DR3 signaling were performed using overexpression systems that can often lead to artifacts. In this chapter we describe how to analyze DR3 downstream events with most attention being paid to endogenous immunoprecipitation.

DOI10.1007/978-1-4939-0669-7_2
Alternate JournalMethods Mol. Biol.
PubMed ID24788169