Detection and differentiation of normal, cancerous, and metastatic cells using nanoparticle-polymer sensor arrays.

TitleDetection and differentiation of normal, cancerous, and metastatic cells using nanoparticle-polymer sensor arrays.
Publication TypeJournal Article
Year of Publication2009
AuthorsBajaj A, Miranda OR, Kim I-B, Phillips RL, Jerry JD, Bunz UHF, Rotello VM
JournalProc Natl Acad Sci U S A
Volume106
Issue27
Pagination10912-6
Date Published2009 Jul 7
ISSN1091-6490
KeywordsAnimals, Biosensing Techniques, Cell Line, Tumor, Humans, Mice, Nanoparticles, Neoplasm Metastasis, Neoplasms, Polymers
Abstract

Rapid and effective differentiation between normal and cancer cells is an important challenge for the diagnosis and treatment of tumors. Here, we describe an array-based system for identification of normal and cancer cells based on a “chemical nose/tongue” approach that exploits subtle changes in the physicochemical nature of different cell surfaces. Their differential interactions with functionalized nanoparticles are transduced through displacement of a multivalent polymer fluorophore that is quenched when bound to the particle and fluorescent after release. Using this sensing strategy we can rapidly (minutes/seconds) and effectively distinguish (i) different cell types; (ii) normal, cancerous and metastatic human breast cells; and (iii) isogenic normal, cancerous and metastatic murine epithelial cell lines.

DOI10.1073/pnas.0900975106
Alternate JournalProc. Natl. Acad. Sci. U.S.A.
PubMed ID19549846