Cloned transgenic calves produced from nonquiescent fetal fibroblasts.

TitleCloned transgenic calves produced from nonquiescent fetal fibroblasts.
Publication TypeJournal Article
Year of Publication1998
AuthorsCibelli JB, Stice SL, Golueke PJ, Kane JJ, Jerry J, Blackwell C, Ponce de León FA, Robl JM
JournalScience
Volume280
Issue5367
Pagination1256-8
Date Published1998 May 22
ISSN0036-8075
KeywordsAnimals, Animals, Genetically Modified, Blastocyst, Cattle, Cell Aging, Cell Division, Cell Nucleus, Cells, Cultured, Clone Cells, Cloning, Organism, Embryo Transfer, Female, Fetus, Fibroblasts, G1 Phase, Male, Nuclear Transfer Techniques, Oocytes, Transfection, Transgenes
Abstract

An efficient system for genetic modification and large-scale cloning of cattle is of importance for agriculture, biotechnology, and human medicine. Here, actively dividing fetal fibroblasts were genetically modified with a marker gene, a clonal line was selected, and the cells were fused to enucleated mature oocytes. Out of 28 embryos transferred to 11 recipient cows, three healthy, identical, transgenic calves were generated. Furthermore, the life-span of near senescent fibroblasts could be extended by nuclear transfer, as indicated by population doublings in fibroblast lines derived from a 40-day-old fetal clone. With the ability to extend the life-span of these primary cultured cells, this system would be useful for inducing complex genetic modifications in cattle.

Alternate JournalScience
PubMed ID9596577