ADAM13 cleavage of cadherin-11 promotes CNC migration independently of the homophilic binding site.

TitleADAM13 cleavage of cadherin-11 promotes CNC migration independently of the homophilic binding site.
Publication TypeJournal Article
Year of Publication2015
AuthorsAbbruzzese G, Becker SF, Kashef J, Alfandari D
JournalDev Biol
Date Published2015 Jul 21
ISSN1095-564X
Abstract

The cranial neural crest (CNC) is a highly motile population of cells that is responsible for forming the face and jaw in all vertebrates and perturbing their migration can lead to craniofacial birth defects. Cell motility requires a dynamic modification of cell-cell and cell-matrix adhesion. In the CNC, cleavage of the cell adhesion molecule cadherin-11 by ADAM13 is essential for cell migration. This cleavage generates a shed extracellular fragment of cadherin-11 (EC1-3) that possesses pro-migratory activity via an unknown mechanism. Cadherin-11 plays an important role in modulating contact inhibition of locomotion (CIL) in the CNC to regulate directional cell migration. Here, we show that while the integral cadherin-11 requires the homophilic binding site to promote CNC migration in vivo, the EC1-3 fragment does not. In addition, we show that increased ADAM13 activity or expression of the EC1-3 fragment increases CNC invasiveness in vitro and blocks the repulsive CIL response in colliding cells. This activity requires the presence of an intact homophilic binding site on the EC1-3 suggesting that the cleavage fragment may function as a competitive inhibitor of cadherin-11 adhesion in CIL but not to promote cell migration in vivo.

DOI10.1016/j.ydbio.2015.07.018
Alternate JournalDev. Biol.
Refereed DesignationRefereed
PubMed ID26206614