Lisa M. Minter

Lisa M. Minter

Assistant Professor

Graduate Program Director

Animal Biotechnology and Biomedical Sciences

Director, Flow Cytometry Core Facility

Photo of Lisa Minter

Office phone: 413-545-6327

Lab phone: 413-545-2392

Fax: 413-545-1446

Email: lminter [at] vasci [dot] umass [dot] edu

Office location: 427K ISB

Mailing address:

661 N Pleasant St
University of Massachusetts/Amherst
Amherst, MA 01003

Ph.D.: University of Massachusetts, 2001
Postdoctoral Training: UMass, Amherst

Classes:
ANIML SCI 365
- Fundamentals of Veterinary & Biomedical Laboratory Techniques
ANIML SCI 572 - Infection and Immunity

Notch signaling in autoimmune diseases

My area of research focuses on the role Notch signaling plays in autoimmune responses. We are currently investigating how Notch signaling contributes to pathology and disease progression during immune-mediated bone marrow failure (BMF).  We have established two new models of BMF, both of which are highly representative of the human immune-meditated BMF syndrome, Aplastic Anemia. In our first model, we transfer bulk splenocytes from a parental C57BL/6 strain into recipient mice that are the F1 hybrid progeny of a C57BL/6 x BALB/c cross. The result is a robust graft-versus-host response whereby the transferred splenocytes selectively target the bone marrow for destruction. The onset of disease is precipitous and symptoms include loss of repopulating stem cells from the bone marrow and immune cells from the periphery, infiltration of destructive CD4+ and CD8+ T cells into the recipient BM, as well as increased levels of the pro-inflammatory cytokines, interferon-g and TNF in the circulation.

The second model of BMF, our “humanized” model, utilizes newly-derived transgenic NOD/SCID/IL2Rgcnull mice which lack murine B, T and NK cells. These mice are particularly amenable to reconstitution with human hematopoietic stem cells. When we transfer human CD34+ umbilical cord blood stem cells into these mice, the cells find their way to the bone marrow and repopulate the mouse with human immune cells, including functional human CD4+ and CD8+ T cells. Four months later, we can detect up to 35% human cells in the circulation of these reconstituted mice. Furthermore, when we transfer human peripheral blood mononuclear cells into these animals, we can again induce a robust immune response that results in nearly complete loss of cells, including human CD34+ cells, from the bone marrow.

Image of mouse and graph

Minter LM, Osborne BA.  2012.  Canonical and Non-Canonical Notch Signaling in CD4(+) T Cells.. Current topics in microbiology and immunology.
Minter LM, Osborne BA.  2012.  Notch and the survival of regulatory T cells: location is everything!. Science signaling. 5(234):pe31.
Das P, Verbeeck C, Minter L, Chakrabarty P, Felsenstein K, Kukar T, Maharvi G, Fauq A, Osborne BA, Golde TE.  2012.  Transient pharmacologic lowering of Aß production prior to deposition results in sustained reduction of amyloid plaque pathology.. Molecular neurodegeneration. 7(1):39.
Keerthivasan S, Suleiman R, Lawlor R, Roderick J, Bates T, Minter L, Anguita J, Juncadella I, Nickoloff BJ, Le Poole IC et al..  2011.  Notch signaling regulates mouse and human Th17 differentiation.. J Immunol. 187(2):692-701.
Cho OH, Shin HM, Miele L, Golde TE, Fauq A, Minter LM, Osborne BA.  2009.  Notch regulates cytolytic effector function in CD8+ T cells.. Journal of immunology (Baltimore, Md. : 1950). 182(6):3380-9.
Joshi I, Minter LM, Telfer J, Demarest RM, Capobianco AJ, Aster JC, Sicinski P, Fauq A, Golde TE, Osborne BA.  2009.  Notch signaling mediates G1/S cell-cycle progression in T cells via cyclin D3 and its dependent kinases.. Blood. 113(8):1689-98.
Samon JB, Champhekar A, Minter LM, Telfer JC, Miele L, Fauq A, Das P, Golde TE, Osborne BA.  2008.  Notch1 and TGFbeta1 cooperatively regulate Foxp3 expression and the maintenance of peripheral regulatory T cells.. Blood. 112(5):1813-21.
Osborne BA, Minter LM.  2007.  Notch signalling during peripheral T-cell activation and differentiation.. Nature reviews. Immunology. 7(1):64-75.
Shin HM, Minter LM, Cho OH, Gottipati S, Fauq AH, Golde TE, Sonenshein GE, Osborne BA.  2006.  Notch1 augments NF-kappaB activity by facilitating its nuclear retention.. The EMBO journal. 25(1):129-38.
Minter LM, Turley DM, Das P, Shin HM, Joshi I, Lawlor RG, Cho OH, Palaga T, Gottipati S, Telfer JC et al..  2005.  Inhibitors of gamma-secretase block in vivo and in vitro T helper type 1 polarization by preventing Notch upregulation of Tbx21.. Nature immunology. 6(7):680-8.
Minter LM, Osborne BA.  2003.  Cell death in the thymus--it' s all a matter of contacts.. Seminars in immunology. 15(3):135-44.
Jerry JD, Minter LM, Becker KA, Blackburn AC.  2002.  Hormonal control of p53 and chemoprevention.. Breast Cancer Res. 4(3):91-4.
Jerry JD, Minter LM, Becker KA, Blackburn AC.  2002.  Hormonal control of p53 and chemoprevention.. Breast cancer research : BCR. 4(3):91-4.
Minter LM, Dickinson ES, Naber SP, Jerry JD.  2002.  Epithelial cell cycling predicts p53 responsiveness to gamma-irradiation during post-natal mammary gland development.. Development (Cambridge, England). 129(12):2997-3008.
Name Phone Office
Chandiran , Karthik MCB Graduate Student 413-545-2392 470 ISB
Chikkegowda , Pavitra ABBS Graduate Student 413-545-2392 470 ISB
Kuksin , Christina ABBS Graduate Student 413-545-2392 470 ISB
Ozay , Emrah IlkerMCB Graduate Student 413-545-2392 470 ISB
Torres , Joe AMCB Graduate student 413-545-2392 470 ISB
Former Lab Personnel